mren1 duoset elisa  (Bio-Techne corporation)

Journal: Hypertension

Article Title: A low salt diet and circadian dysfunction synergize to induce angiotensin II-dependent hypertension in mice

doi: 10.1161/HYPERTENSIONAHA.115.06194

Figure Lengend Snippet: (A) The level of renin expression in kidney was measured by qRT-PCR. Kidney renin expression in Per-TKO was increased over 2-fold compared to WT in conditions of low salt. (B) Plasma renin concentration was determined with an ELISA kit (Mouse Renin 1 DuoSet, R&D Systems). Per-TKO mice display a significant elevation in plasma renin (*p<0.05, Unpaired Student t test). (C) In aorta, renin expression was determined by Western blotting which also showed a significant increase in renin in low salt-treated Per-TKO mice. Shown as western blot of three representative WT and three representative Per-TKO mice and densitometry (WT n=5, KO n=3, *p<0.05, Unpaired Student t test). Aorta (D) and Kidneys (E) were harvested from WT and Per-TKO mice in LD at 6 hour intervals for 42 hours under normal diet conditions. Aorta and kidney AT1 expression show a phase shift with higher daytime expression in Per-TKO versus WT mice. (F) Relative mRNA expression by Q-PCR of control and Per2 with siRNA transfection (50 nmol). (G) Western blotting shows Per2 knockdown decreases AT1 receptor expression in HASMCs and is further decreased with 24 hours of angiotensin II treatment (100 nmol/L), quantified by densitometry (n=3 per group, *p<0.05, 1-way ANOVA).

Article Snippet: Kidney renin expression in Per-TKO was increased over 2-fold compared to WT in conditions of low salt. ( B ) Plasma renin concentration was determined with an ELISA kit (Mouse Renin 1 DuoSet, R&D Systems).

Techniques: Expressing, Quantitative RT-PCR, Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot, Control, Transfection, Knockdown